Specimens, cultures, ribosomal and other genomic DNA sequences collected from Antarctica
The fieldwork involved collection of fertile lichens from a range of sites across the Antarctic Peninsula and isolation of the lichen-forming fungi into pure culture in a laboratory at Rothera. Approximately 5,600 monospore cultures were isolated, including B frigida. Approximately 400 thalli of Usnea species, and 3 O. frigida thalli have also been collected for whole thallus analysis. Logarithmic sampling transects of B frigida were conducted at Rothera (2 transects) and on Anchorage Island (one transect) to examine the genetic variation and geographic variation. All thalli of B frigida collected from the transects were successfully used to generate viable spores from four individual apothecia from each thallus. 16 spores were subcultured and maintained from each apothecium.
Rothera, Usnea, base sequences, frigida, lichen, phylogenetics
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|Reference:||Fabian A. Seymour, Peter D. Crittenden, Nora Wirtz, Dag O. Ovstedal, Paul S. Dyer, H. Thorsten Lumbsch, 2007. Phylogenetic and morphological analysis of Antarctic lichenforming Usnea species in the group Neuropogon. Antarctic Science 19 (1), 71-82 (2007) DOI: 10.1017/S0954102007000107 71.
Fabian A. Seymour, Peter D. Crittenden, Matthew J. Dickinson, Mathieu Paoletti, Dolores Montiel, Lily Cho, Paul S. Dyer, 2005.
Breeding systems in the lichen-forming fungal genus Cladonia. Fungal Genetics and Biology 42 (2005) 554-563.
Project ref: AFI 02/47 - Genetic variation in Antarctic lichens
|Quality:||All data were subjected to QC
The sampling programme was very successful and has yielded a substantial collection
All sequences that did not satisfy the appropriate QC standards were discarded
|URL Content Type||GET DATA|
|URL Content Type||RELATED DATA SET DESCRIPTION|
|Detailed Location||Leonie, Lagoon, Charcot and Adelaide Islands, Antarctic Peninsula|
|Detailed Location||Sky-Blu Nunatak|
|Location||South Atlantic Ocean|
|Detailed Location||Falkland Islands|
|Data Storage:||Voucher specimens deposited at British Museum with following specimen numbers:
F. Seymour 79, 125 for U. antarctica;
F. Seymour 211, 214 for U. aurantiaco-atra;
F. Seymour 269, 274 for U. sphacelata;
F. Seymour 284, 288, 290, 293 for U. subantarctica.
Specimens also deposited at CABI and Egham (IMI) with the following reference numbers:
Voucher specimens of X. floerkeana, C. galindezii, C. portentosa - CABI Bioscience (UK) at Egham (IMI), respectively:
IMI 391500, IMI 391496, and IMI 391504.
Replicate axenic cultures of each fungus at CABI Bioscience:
C. Xoerkeana, IMI 391501, IMI 391502, IMI 391503; C. galindezii, IMI 391497, IMI 391498, and IMI 391499; C. portentosa IMI 391505, IMI 391506, and IMI 391507.
Base sequences for 5 main groups deposited in Genbank with following accessions numbers:
DQ235496, DQ767952-DQ767965 and EF179795-EF179806 (ITS region); DQ658417, EF179782-EF179794 and EF193046-EF193058 (RPB1) .
Buellia frigida and Ochrolechia frigida specimens and data are currently managed by Nottingham University.
|Access Constraints:||B. frigida and Ochrolechia frigida specimens are not currently accessible. Requests for access should be made via the place of deposit (see storage).|
|Use Constraints:||These data are governed by NERC data policy.|